Stephanie was Orphen's old partner and initially a man but was severely injured and while using the rest of his magic to cast a healing spell, made some "modifications", and became the woman Stephanie. She avenges Chinkyuu's death by trying to fight Hakufu at Toutaku's place, ending up committing murder suicide with Toutaku with a point-blank chi blast. In the manga, Akira has no romantic feelings for Shirogane while Shirogane is in love with King Ryuko. In the carnival it was revealed that Shizuru is Natsuki's most important person but because of Shizuru's psychotic rampage Natsuki began to doubt her feelings. Yivo is a planet-sized alien with no determinable gender. Mitsuka often strips down to her lingerie during classes, makes her students read erotic stories,  openly asks about their sex lives  and flirts with them.
Linda saved Francine from the Lady Bugs, a social group for women who cheat on their husbands, by kissing her.
List of animated works with LGBT characters
Dee Laytner and Ryo Maclean are cops and partenrs who work in New york. He states that if a relationship with her didn't work out, it won't work out with any other girl. He was thought to be a homosexual by Peter. He is seen having fantasies about Suetake, and not much else. Ayano is in love with Kyouko but is often not too honest with her feelings for her, always referring to her by her full name and treating her with acts of hostility, despite not wanting do so on purpose. Due to her obsession with Squid Girl, her Dog, Alex often tries to gain her attention, through various means, and will often bite Squid Girl, in anger, despite Squid Girl's own irritation with Sanae's obsession. Ran is shown to be only interested in girls and hates Taketo, but this changes after Taketo helps rescue Fukune from Beni Botan.
She and Neviril had been a pair, and romantically partnered, ever since Neviril first joined Chor Tempest. Kaikaina "Sister" Grif is an openly bisexual woman, previously being bi-curious, who appears as a main character in Season 5 and The Shisno Paradox. In the anime adaptation, she serves as a subordinate for Yukina, whose mission is to observe and eventually kidnap Miharu. Ryomo is eventually relieved of her duties to be appointed as an apprentice strategist to Sonken, whom she appears to have developed a crush on during their time spent together it was implied that Sonken also feels this way. After Hazumu's transformation into a girl, Yasuna wastes no time trying to rectify her mistake and confesses her love to Hazumu finally, but Hazumu is very confused by this and does not know what to do, especially since her physical change. Robin Brenner calls the character "one of the most realistic and accepting portrayals of a transgender character in manga.